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Graphpad student6/12/2023 ![]() Thanks in advance for any guidance, ideas, and/or discussion. ![]() My question is: How do I take the ‘intrinsic activity’ of the enzyme into account (if I need to at all)? Can anyone recommend an equation and appropriate software that can do this? We currently have SigmaPlot, GraphPad, and Enzfitter (for the double substrate inhibition model). We’ve run a full course of analyses, varying the NADH as well as the substrate and we're now trying to fit the data to an appropriate equation. Biomedical Graduate Studies (BGS) serves as the academic home within the University of Pennsylvania for roughly 700 graduate students pursing a PhD in the. Pros: GraphPad Prism offers an intuitive and easy-to-use interface that simplifies the process of data analysis for users of all skill levels.Prism provides a wide range of statistical tests, including nonlinear regression, ANOVA, and t-tests, making it a versatile tool for data analysis. The intrinsic activity introduces a baseline velocity and is complicating our kinetic analyses a bit. Complicating it even further is a noted double substrate inhibition pattern (apparently not uncommon with ping-pong mechanism enzymes). GraphPad Prism has a truly user-friendly interface that allows data administration and analysis databases, as well as the creation of reports using graphs. Of course, upon addition of substrate, the velocity of the reaction increases and NADH is consumed more rapidly. ![]() ![]() I’m working with a rather complicated ping pong, bi-bi double displacement reductase enzyme that oxidizes NADH and then reduces a substrate. Interestingly, the enzyme has intrinsic NADH oxidase activity in the absence of the substrate. ![]()
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